expression vector plpcx (TaKaRa)
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expression vector plpcx
Expression Vector Plpcx, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plpcx+expression+vector/LRCX+Retroviral+Vector+Set/pm40890526-204-7-10
Average 95 stars, based on 26 article reviews
Expression Vector Plpcx, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plpcx+expression+vector/LRCX+Retroviral+Vector+Set/pm40890526-204-7-10
Average 95 stars, based on 26 article reviews
expression vector plpcx - by Bioz Stars,
2026-10
95/100 stars
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Construct:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Sequencing:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: ZNRD1 (zinc ribbon domain-containing 1) is a host cellular factor that influences HIV-1 replication and disease progression. Article Snippet: After reverse transcription with hexamers (Expand RT; Roche), ZNRD1 was amplified using the following primers, which introduced, respectively, EcoRI and ClaI (shown in boldface type): 5′-GAATTCgccaccATGTCTGTCATGGACCTCGCCAATAC-3′ and 5′-ATCGATggctcaAGAGTCTTCCTTCTCCTGGAACTTG-3′. .. PCR products were digested with EcoRI and ClaI, introduced into the Generated:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. Generation and Expression of Membrane-bound PLA2R1 Deletion Mutants We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Polymerase Chain Reaction:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. Generation and Expression of Membrane-bound PLA2R1 Deletion Mutants We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: ZNRD1 (zinc ribbon domain-containing 1) is a host cellular factor that influences HIV-1 replication and disease progression. Article Snippet: After reverse transcription with hexamers (Expand RT; Roche), ZNRD1 was amplified using the following primers, which introduced, respectively, EcoRI and ClaI (shown in boldface type): 5′-GAATTCgccaccATGTCTGTCATGGACCTCGCCAATAC-3′ and 5′-ATCGATggctcaAGAGTCTTCCTTCTCCTGGAACTTG-3′. .. PCR products were digested with EcoRI and ClaI, introduced into the Clone Assay:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. Generation and Expression of Membrane-bound PLA2R1 Deletion Mutants We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Expressing:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: A proximity ligation assay using transiently transfected, epitope-tagged proteins: application for in situ detection of dimerized receptor tyrosine kinases. Article Snippet: Methods and materials Expression constructs Human wild-type EGFR-encoding cDNA (lacking stop codon) was PCR-amplified from the pGEM7zf(-):EGFRwt (Promega, Madison, WI, USA) shuttle vector (primer sequences available in Supplementary Table S1) and cloned into pCR2.1-TOPO using the TOPO-TA system (Invitrogen, Burlington, ON, Canada) for sequence verification. .. EGFRwt-pCR2.1-TOPO was SacII-digested and blunt-end–cloned into the HindIII site of Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. Generation and Expression of Membrane-bound PLA2R1 Deletion Mutants We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Article Title: ZNRD1 (zinc ribbon domain-containing 1) is a host cellular factor that influences HIV-1 replication and disease progression. Article Snippet: After reverse transcription with hexamers (Expand RT; Roche), ZNRD1 was amplified using the following primers, which introduced, respectively, EcoRI and ClaI (shown in boldface type): 5′-GAATTCgccaccATGTCTGTCATGGACCTCGCCAATAC-3′ and 5′-ATCGATggctcaAGAGTCTTCCTTCTCCTGGAACTTG-3′. .. PCR products were digested with EcoRI and ClaI, introduced into the Plasmid Preparation:Article Title: Clinical Relevance of Domain-Specific Phospholipase A2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: PLA2R1 deletion constructs were designed as previously described.11 As a first step, the wild-type human PLA2R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA2R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Article Title: ZNRD1 (zinc ribbon domain-containing 1) is a host cellular factor that influences HIV-1 replication and disease progression. Article Snippet: After reverse transcription with hexamers (Expand RT; Roche), ZNRD1 was amplified using the following primers, which introduced, respectively, EcoRI and ClaI (shown in boldface type): 5′-GAATTCgccaccATGTCTGTCATGGACCTCGCCAATAC-3′ and 5′-ATCGATggctcaAGAGTCTTCCTTCTCCTGGAACTTG-3′. .. PCR products were digested with EcoRI and ClaI, introduced into the Mutagenesis:Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the Proximity Ligation Assay:Article Title: Clinical Relevance of Domain-Specific Phospholipase A 2 Receptor 1 Antibody Levels in Patients with Membranous Nephropathy Article Snippet: 11 As a first step, the wild-type human PLA 2 R1 cDNA (GenBank NM007366) was cloned into the pGEM-Teasy vector (Promega, Madison, WI). .. The PLA 2 R1 deletion constructs, to which an HA-tag was fused to the C terminus of the coding sequence, were then generated by PCR and cloned into the Membrane:Article Title: Epitope Spreading of Autoantibody Response to PLA2R Associates with Poor Prognosis in Membranous Nephropathy Article Snippet: .. Generation and Expression of Membrane-bound PLA2R1 Deletion Mutants We generated the series of human PLA2R1 deletion mutants by PCR and cloned them into the |